One of the most significant shifts in emphasis in recent years on the MCAT was the announcement by AAMC of greater emphasis on genetics and molecular biology on the exam. This is a shift that practically guarantees the presence of such a passage on your exam. More than ever, students need to be comfortable with discussion of the basic repertoire of techniques in molecular genetics. One such technique is in situ hybridization. In this technique a labeled complementary DNA or RNA strand is used to localize a specific DNA or RNA sequence in a portion or section of tissue (in situ). When a labeled DNA or RNA strand is used in this way, it is refered to as a probe. Probes may be labeled with radioisotope, antigen, enzyme, or fluorescent dye, to be detected respectively through associated schema such as autoradiography or immunohistochemical methods, for example. Historically, the techniques of in situ hybridization have been very important in chromosomal localization experiments.












The WikiPremed MCAT Course is a comprehensive course in the undergraduate level general sciences. Undergraduate level physics, chemistry, organic chemistry and biology are presented by this course as a unified whole within a spiraling curriculum. Please read our policies on Privacy and Shipping & Returns.  Contact Us. MCAT is a registered trademark of the Association of American Medical Colleges, which does not endorse the WikiPremed Course. WikiPremed offers the customers of our publications or our teaching services no guarantees regarding eventual performance on the MCAT.


Creative Commons License
WikiPremed is a trademark of Wisebridge Learning Systems LLC. The work of WikiPremed is published under a Creative Commons Attribution NonCommercial ShareAlike License. There are elements of work here, such as a subset of the images in the archive from WikiPedia, that originated as GNU General Public License works, so take care to follow the unique stipulations of that license in printed reproductions.